glut 3 (Proteintech)

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Proteintech glut 3
Glut 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glut+3/GLUT3+Antibody/bio_rxiv__2025__09__14__676169-178-18-19
Average 94 stars, based on 115 article reviews

glut 3 - by Bioz Stars, 2026-09

94/100 stars

Images

Staining:

Article Title: Inhibition of hypoxia-inducible factor 1 by acriflavine renders glioblastoma sensitive for photodynamic therapy.
Article Snippet: The antibodies were attenuated with antibody diluents (Scytek, Logan, UT). .. HIF1a, GLUT-1, GLUT-3, HK2 (rabbit anti-human, polyclonal, Proteintech, China) were stained with BrightVision HRP-labeled anti-rabbit polymer (Immunology) and BrightDAB. ..

Polymer:

other:

Article Title: 16p11.2 haploinsufficiency reduces mitochondrial biogenesis in brain endothelial cells and alters brain metabolism in adult mice.
Article Snippet: After a final wash (TBST, 33 10min), the proteins were detected by enhanced chemiluminescence (ECL; 1⁄4, Fisher Scientific, #34579) and imaged with the Odyssey Imaging system.

Incubation:

Article Title: 16p11.2 haploinsufficiency reduces mitochondrial biogenesis in brain endothelial cells and alters brain metabolism in adult mice.
Article Snippet: Immunoblotting Fifteen (15) mg of protein were loaded into the wells of SDS-acrylamide gels (GX Stain-free FastCast 12% Biorad gels, Bio-Rad, ON, CAN, #1610184) and separated by a constant current of 100 V for 120 min in running buffer (SDS 35mM, Tris 250mM, Glycine 1865mM). .. The membranes were then blocked with 5% skim milk in 1M PBS for 1 h at room temperature and incubated with primary antibodies raised again either MCT-1 (1:200, Alomone, #AMT-011), GLUT-1 (1:1000, Novus-Bio, #NB110-39113), GLUT-3 (1:2000, ProteinTech, 20403-1-AP), hexokinase-1 (1:1500, Abcam, ab150423), hexokinase-2 (1:1500, Abcam, ab209847), GSK3b (1:3000, Cell Signaling, 12456), AMPK (1:1000, Cell Signaling, 2532), phospho-AMPK (1:800, Cell Signaling, 2535), DRP1 (1:2000, BD Biosciences, 611113), MFN1 (Abcam, ab126575, 1:1000), MFN2 (1:1000, Abcam, ab56889), OPA1 (1:2000, Abcam, ab42364), tubulin (1:5000, Sigma, T6199), PGC-1a (1:1500, Millipore, ST1202), GAPDH 16 Cell Reports 42, 112485, May 30, 2023 (1:10000, ProteinTech, 60004-1-Ig) or Vinculin (1:5000, Abcam, ab129002) in TBST (Tris 50mM, NaCl 150mM, Tween 20 1% v/v) overnight at 4 C. The membranes were then washed with TBST (3 3 10 min) and incubated at room temperature for 1 h with the secondary antibody (1/10,000, Fisher Scientific, #PR-W4011), also diluted in TBST. .. After a final wash (TBST, 33 10min), the proteins were detected by enhanced chemiluminescence (ECL; 1⁄4, Fisher Scientific, #34579) and imaged with the Odyssey Imaging system.

Pyrolysis Gas Chromatography:

Article Title: 16p11.2 haploinsufficiency reduces mitochondrial biogenesis in brain endothelial cells and alters brain metabolism in adult mice.
Article Snippet: Immunoblotting Fifteen (15) mg of protein were loaded into the wells of SDS-acrylamide gels (GX Stain-free FastCast 12% Biorad gels, Bio-Rad, ON, CAN, #1610184) and separated by a constant current of 100 V for 120 min in running buffer (SDS 35mM, Tris 250mM, Glycine 1865mM). .. The membranes were then blocked with 5% skim milk in 1M PBS for 1 h at room temperature and incubated with primary antibodies raised again either MCT-1 (1:200, Alomone, #AMT-011), GLUT-1 (1:1000, Novus-Bio, #NB110-39113), GLUT-3 (1:2000, ProteinTech, 20403-1-AP), hexokinase-1 (1:1500, Abcam, ab150423), hexokinase-2 (1:1500, Abcam, ab209847), GSK3b (1:3000, Cell Signaling, 12456), AMPK (1:1000, Cell Signaling, 2532), phospho-AMPK (1:800, Cell Signaling, 2535), DRP1 (1:2000, BD Biosciences, 611113), MFN1 (Abcam, ab126575, 1:1000), MFN2 (1:1000, Abcam, ab56889), OPA1 (1:2000, Abcam, ab42364), tubulin (1:5000, Sigma, T6199), PGC-1a (1:1500, Millipore, ST1202), GAPDH 16 Cell Reports 42, 112485, May 30, 2023 (1:10000, ProteinTech, 60004-1-Ig) or Vinculin (1:5000, Abcam, ab129002) in TBST (Tris 50mM, NaCl 150mM, Tween 20 1% v/v) overnight at 4 C. The membranes were then washed with TBST (3 3 10 min) and incubated at room temperature for 1 h with the secondary antibody (1/10,000, Fisher Scientific, #PR-W4011), also diluted in TBST. .. After a final wash (TBST, 33 10min), the proteins were detected by enhanced chemiluminescence (ECL; 1⁄4, Fisher Scientific, #34579) and imaged with the Odyssey Imaging system.

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